Coding

Part:BBa_K3301001:Design

Designed by: Maria Lyons, Hailey Ho, Vina Chen, Zoe Hsieh, Josh Dayie   Group: iGEM19_Baltimore_BioCrew   (2019-10-15)


Codon optimized B. theta gene BT2268


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1178
    Illegal BsaI.rc site found at 1634
    Illegal SapI site found at 1553


Design Notes

Removed initial signal sequence. Codon optimized for E. coli.


Source

Synthesized by IDT

References